Prestained protein ladder western blot
Find prestained ladders compatible for western blotting.
Quality Control :ĥ μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. Find the right protein ladder (molecular weight markers) for your protein electrophoresis needs. Contents :Īpproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25☌), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) glycerol.
The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated by SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). BenchMark Pre-Stained Protein Ladder contains 10 proteins ranging in apparent molecular weight from 6 to 180 kDa for use in monitoring electrophoresis or transfer from gel to membrane in western blotting. The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.